Screening of 12 species of Shorea and 2 species of Anisoptera from the forest of Pasir Mayang, Jambi with brine shrimp (Anemia salina) lethality bioassay showed that Shorea gibosa and Anisoptera marginata have sufficient activity for further investigation. Bioassay-guided fractionation of the active extract of S. gibosa led to the isolation of stigmasterol and the shoreaphenol. Bioassay-guided fractionation of the active extract of A. marginata resulted in the isolation of lupenone and 3-methoxy-4-hydroxybenzoic acid O-p-C glucopyranoside as the bioactive compound.