Malaria parasites develop in erythrocytes and naturally-acquired immune responses can result in either the elimination of the parasites or a persistent response. The cytokines are responsible for all the symptoms, pathological alterations and the outcome of the infection depending on the reciprocal regulation of the pro inflammatory (TNF-α) and anti-inflammatory (IL-10) cytokines. The aim of this study was to describe the level of IL-10 and TNF- α on malaria infection, using an analytic laboratory cross-sectional design. The serum levels of the cytokines TNF- α and IL-10 from 50 patients were evaluated by indirect ELISA. The results revealed that increased levels of IL-10 and TNF-α among respondents without clinical symptoms of malaria were higher compared to respondents with clinical symptoms of P. falciparum and P. vivax. Statistically, there was no significant association between clinical symptoms with increased cytokine IL-10 and TNF-α.The ratio of TNF-α / IL-10 in respondents with clinical symptoms and without clinical symptoms indicated that the respondent without clinical symptoms was higher than that of clinical symptoms. The study concluded that molecular basis of immune response of patients in the study site is still very good because of reciprocal response between pro-inflammatory cytokines and anti-inflammatory.