Daya Saing Kawin Nyamuk Jantan Steril (Culex Quinquefasciatus) Skala Laboratorium: Studi Awal Penggunaan Teknik Serangga Mandul Dalam Pengendalian Vektor Filariasis Limfatik Di Kota Pekalongan

Ramadhani, Tri • Hadi, Upik Kusuma • Soviana, Susi • Irawati, Zubaidah • Sunaryo, Sunaryo

Abstract

Culex quinquefasciatus is the main vector of limfatic filariasis in Pekalongan City. Sterile Insect Tehnique could be an alternative vector control efforts to eliminate filariasis. The success of this technique is depend on the ability of laboratory-reared sterile males with the wild-type females. Indicator of SIT Aplication is determined by the value of the mating competitiveness and sterility to Culex quinquefasciatus (Diptera:Culicidae). The design of the research is an experimental. Gamma irradiation on the pupae (age . 15 hours) with the doses of 0 Gy, 60 Gy, 65 Gy,70 Gy, 75 Gy and 80 Gy in BATAN Jakarta. Male mosquitoes which emerged from the pupa then matting with a normal female. This research observed the mean of females laying eggs ,fecundity, fertility and mating competitiveness. This experimental research was conducted in the laboratory and the data were analyzed by ANOVA.The result showed that irradiation at the trial doses had an effect on fertility of Culex quinquefasciatus, but not had significant effect on fecundity and mating competitiveness . A dose of 70 Gy is the optimum dose with a fertility rate of 1.8% (sterility 98.2%) and C indexs 0,568 can be recommended for futher semi field assays. The number of sterile males were six times compared with the wild population to increase the chances of mating with wild-type females. Culex quinquefasciatus merupakan vektor utama filiriasis limfatik di Kota Pekalongan. Teknik Serangga Mandul dapat menjadi alternatif upaya pengendalian vektor filariasis. Keberhasilan teknik ini tergantung kemampuan jantan mandul untuk kawin dengan betina alam di laboratorium. Indikator aplikasi Teknik Serangga Mandul (TSM) ditentukan dengan nilai daya saing kawin dan sterilitas. Desain penelitian yang digunakan adalah eksperimental. Nyamuk Culex quinquefasciatus berasal dari galur lokal kota Pekalongan. Iradiasi gamma pada pupa (umur >15 jam) dengan dosis uji 0,60,65,70,75 dan 80 Gy di BATAN Jakarta. Nyamuk jantan yang muncul dari pupa kemudian di kawinkan dengan betina normal. Hasil perkawinan diamati jumlah nyamuk betina yang mampu bertelur, fertilitas, fekunditas dan daya saing kawin. Data dianalisis dengan uji ANOVA. Hasil penelitian menunjukkan iradiasi berpengaruh terhadap fertilitas akan tetapi tidak dengan fekunditas dan daya saing kawin. Dosis 70 Gy merupakan dosis optimum dengan tingkat fertilitas 1,8% (sterilitas 98,2%) dan C indeks 0,586 dapat direkomendasikan untuk uji lanjut semi lapang. Banyaknya jantan steril yang dilepas enam kali dari populasi alam untuk memperbesar peluang kawin dengan betina normal.

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Jurnal Litbang Pengendalian Penyakit Bersumber Binatang Banjarnegara

BALABA is a peer-reviewed scientific journal that publishes original research and literature revi... see more