Belitung is one of potential food crops, which has a lot of benefit. Collection of belitung germplasm conserved in the field is sometimes lost or damaged by biotic and abiotic stress. Therefore, in vitro conservation is very important to do to minimize loss of collection. The purpose of this research was to study the effect of benzylaminopurine (BAP) on in vitro shoot multiplication of belitung and to study the influences of osmoticum (mannitol) and retardant (paclobutrazol/PBZ) to the growth of belitung culture. Experimental design used in this research was Randomized Completely Design with three replications. Shoots from sucker were used as explant. In this study of shoot multiplication, the treatment medium was MS + BAP (0, 1, 2, 3 mg/l). In the study of conservation, the treatment medium was MS + 30 g/l sucrose, &frac12; MS + 30 g/l sucrose, MS + 15 g/l sucrose, MS + mannitol (20, 40, 60 g/l), and MS + PBZ (1, 2, 3 mg/l). The result showed that the addition of BAP into MS medium increased number of shoot. The best medium for micropropagation of belitung germplasm was MS + BAP 3 mg/l. The highest number of shoot (7.6 shoots) was produced by MS + BAP 3 mg/l medium. MS + mannitol (20 g/l and 40 g/l) and MS + PBZ (1, 2, 3 mg/l) medium could reduce the plant growth. MS + PBZ 2 mg/l was the best medium for belitung conservation. This media could reduce plant growth during 8 months conservation period and prolong subculture interval.