Cholera is one of health problems in Indonesia. The high fatality rate is caused by the enterotoxin. The symptoms are dehydration, electrolyte loss and increased blood acidity, so that in severe case can cause death. Diarrheal disease cholera can lead to an out break. Conventional techniques such as culture, isolation and identification are the gold standard to diagnose V.cholerae, but required considerable time and adequate equipment. thus rapid diagnostic test plays an important role to detect V.cholerae.. The use of rapid diagnostic test can be need as the initial examination, so that preventive actions can be conducted . Rapid diagnostic tests are performed by various methods such can be used directly in the field and the results can be identified quickly, so it can replace the conventional techniques. The advantages of rapid test are quick, easy, inexpensive, practice ; thus it can be used in the field. Rapid test has a sensitivity of 94-100% and a specificity of 84-100% when compared with the conventional technique.