. Agisimanto, D. and A. Supriyanto. 2007. Genetic Diversity of Pummelo Based on Primer Random Amplified Polymorphic DNA. Genetic variablitiy is needed to understand relationship among 18 pummelo varieties in Indonesia. The objective of the research was to characterize genetic diversity of some pummelo varieties in Indonesia based on RAPD primer. Leaves from young flush, 20-25 days, were extracted and amplified by 15 RAPD primers. Bands of DNA were scored based on their presence and absence. Two primers of OPN 14 and OPN 16 were selected to visualize band pattern of pummelo varieties. At least, 3 groups of pummelos were clustered that showed closely relationship and highly similarity by place of origin and fruit characteristics.