. The experiment was aimed to find out the role of five arbuscular mycorrhizal fungus isolates in increasing nutrient uptake and biomass production on seedlings of two cultivars of papaya. The research was conducted at a screen house of Agri- culture Faculty, Padjadjaran University, Bandung, from August 2001 until January 2002. This experiment was laid on a randomized block design of factorial pattern with three replications. The first factor was the kind of mycorrhizal iso- late, control, Glomus etunicatum, Glomus manihotis, Gigaspora margarita, Acaulospora tuberculata, and Scutellospora heterogama, and the second factor was papaya cultivars of dampit and sari rona. The results showed that root infection level of papaya seedlings at two months after inoculation was influenced by interactions between mycorrhizal isolates and papaya cultivars. Acaulospora tuberculata, G. etunicatum, and Gi. margarita isolates showed very high root infection level which were higher than 76% for both papaya cultivars, whereas two other mycorrhizal isolates showed lower infection 21.33-59.67%. Nutrient uptake of N,P,K, and shoot dry weight, in auton- omous caused by kinds of mycorrhizal isolates and papaya cultivars. Acaulospora tuberculata and G. etunicatum iso- lates increased total dry weight 1.028% and 1.632% respectively higher than control. Sari rona showed higher nutrient uptake of N, P, K, and shoot dry weight than dampit. The mycorrhizal application for papaya has good prospect, but further studies are required to determine the fertilizer efficiency.