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description Journal article public Jurnal Hortikultura

Uji Kepekaan Antiserum Poliklonal Untuk Deteksi Cepat CMV Dengan Metode ELISA Tidak Langsung Pada Tanaman Anthurium

I. B. Rahardjo, E. Diningsih, Y. Sulyo
Published 30 September 2008

Abstract

. Rahardjo, I.B., E. Diningsih, and Y. Sulyo. 2008. Sensitivity Test of Cucumber Mosaic Virus Polyclonal Antisera for Rapid Detection of CMV with Indirect ELISA on Anthurium. Cucumber mosaic virus is one of the major pathogens on some horticulture crops including Anthurium. A rapid detection method should be developed to support the evaluation of initial infection of the virus in crops. A serological method commonly used for rapid detection of plant viruses is ELISA (enzyme-linked immunosorbent assay). The objective of the experiment was to determine the optimum concentration of CMV antiserum for rapid detection on anthurium. The experiment was done in Virological Laboratory of Indonesia Ornament Crops Research Institute in Segunung (1.100 m asl.), from April to July 2005. A polyclonal CMV antiserum had been produced by injections of purified CMV into a rabbit with the concentration of 1 mg/ml each injection from previous research. Antiserum concentration was measured with the spectrophotometer at 280 nm wave length. The sensitivity tests of the antiserum was carried out with indirect ELISA method. The results showed that the optimum concentration of the antiserum and the optimum sample dilution for detection of CMV infected anthurium were 1/1,000 and 1/5, 1/1,000 and 1/10 respectively.

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