. An experiment was aimed to find out the role of five arbuscular mycorrhizal fungus isolates in increasing nutrient uptake and biomass production on two cultivars of papaya seedling. The research was conducted at a screen house of Agricul- ture Faculty, Padjadjaran University, from August 2001 until January 2002. This experiment was laid in Randomized Blocks Design in factorial pattern with three replications. The first factor was the kind of mycorrhizaes isolates, con- trol, Glomus etunicatum, Glomus manihotis, Gigaspora margarita, Acaulospora tuberculata and Scutellospora heterogama, and the second factor was papaya cultivars of Dampit and Sari Rona. The results showed that root infec- tion level of papaya seedlings on two months after inoculation influenced by interactions between mycorrhizaes iso- late and papaya cultivars. Acaulospora tuberculata, G. etunicatum, and Gi. margarita isolates showed very high root infection level which was higher than 76% from both papaya cultivars, whereas two other mycorrhizaes isolate showed the lower infection 21.33-59.67%. Nutrient uptake on N,P,K, and shoot dry weight, in autonomous caused by kinds of mycorrhizaes isolate and papaya cultivars. Acaulospora tuberculata and G. etunicatum isolates increased to- tal dry weight 1.028% and 1.632% respectively higher than control. Sari Rona cultivar showed higher nutrient uptake on N, P, K, and shoot dry weight than Dampit cultivar. The mycorrhizaes application for papaya have good prospect, but there needs further studies to know the effectiveness in fertilizer efficiency.