Dengue Fever in Wonosobo district had been increasing rapidly during 2009. Sub-district Wonosobo andGarung have different altitude characteristic and geographic. Potential transmissions based on altitude haveto be learned, especially in association with mosquitoes as dengue fever's vector. The objectives of thisstudy is to count gonotropic cycle and chance of Ae. aegypti and Ae. albopictus' survival. Spot surveymethod and cross sectional design were used for this study. This study was being held in Pagerkukuhvillage (752-842 m) and Jaraksari village (765-781 m) at Wonosobo sub-district and Gemblengan village(1.166 m) at Garung sub-district. Mosquitoes were trapped in 100 m radius from dengue fever's casereport. Gonotropic cycle of Ae. albopictus in Gemblengan village is longer (3 - 7 days) than Pagerkukuh's(3 - 5 days). Gonotopic cycle's of Ae. aegypti in Jaraksari village lasts for 3 - 5 days. Survival chance ofAe. albopictus in Gemblengan village is higher (0,61-0,81) than Pagerkukuh village (0,54-0,69).Meanwhile, survival chance of Ae. aegypti in Jaraksari village was high (0,86-0,91). It's suggested to prioroutside house PSN (mosquito's nest eradication). Therefore, training for thermal fogging officer outsidehouse PSN is important. It also needs dengue virus' detection to verificate Ae. albopictus as dengue fever'svector.