Dengue Haemorrhagic Fever is still a health problem in the world, including in Indonesia. Ecology ofmosquito-borne dengue fever, especially on the influence of climate on development is essential to predictthe development and success of vector control. A descriptive epidemiological study types and usedquantitative methods and research design was a series of periodic (time-series). The overall researchactivities carried out in September 2010 to April 2011. Secondary data collected were temperature,humidity and rainfall. The results showed that there is a relationship between rainfall and dengue cases (r =0.580) and humidity with the dengue cases (r = 0.412). There was no relationship between the temperatureof the dengue cases in Gunungkidul in 2010 and there was no significant association between rainfall factorwith endemicity status. Based on the results of this study recommended the need for monitoring of climate,especially rainfall patterns that occur in Gunungkidul so predictable increase in dengue cases and furthercontrol measures that can be done on time.