. Diningsih, E., F. Rahmawati, Y. Sulyo, and Darliah. 2009. In Vitro Rooting Method for ProducingFree-Virus Rose Rootstock. The method for producing virus free plantlet of rose rootstock had been obtained inprevious study. In obtaining virus-free seed, availability of appropriate in vitro rooting method is needed. Rootingis one of the important step in tissue culture technique for plant rapid multiplication. The purpose of this experimentwas to obtain the best rooting medium for producing virus-free rose rootstock. The experiment was conducted in theVirology Laboratory of the Indonesian Ornamental Crop Research Institute, Segunung, Cianjur, West Java (1,100 masl.) from January to December 2003. The research was laid in a factorial randomized block design with 3 replications.The first factor was 3 virus-free rose rootstock cultivars (Rosa multiflora, Rosa sp. cv. Multic, and cv. Natal Brior)resulted from the previous experiment. The second factor was 7 compositions of medium (MS+IBA 0.5 mg/l, MS+IBA1.0 mg/l, MS+NAA 0.5 mg/l, MS+NAA 1.0 mg/l, MS+IAA 0.5 mg/l, MS+IAA 1.0 mg/l, and MSO (without PGR)).The results showed that the best rooting medium for producing virus-free rose rootstock was MS+IBA 0.5 mg/l.