Pemurnian Parsial Dan Karakterisasi Amilase Dari Bakteri Laut Arthrobacter Arilaitensis LBF-003

Dianti Rahmasari • Wijanarka Wijanarka • Sri Pujiyanto • Nanik Rahmani • Yopi Yopi
Journal article Indonesian Journal of Biology • 2016

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(Bahasa Indonesia, 8 pages)


Starch is an abundant carbon source in nature, and ?-amylase (1, 4-?-D-glucanohydrolase; EC, which hydrolyzes ?-1, 4-glucosidic linkage in starch-related molecules. Microbe ?-amylase production is a hydrolytic enzyme and one ofinterest in its microbial production has increased dramatically due to its wide spread use in food, textile, baking anddetergent industries in recent years. Here we report ?-amylase from marine bacterium which was purified andcharacterized, as well as analyzed its hydrolysis product on starch. The enzyme of Arthrobacter arilaitensis partiallypurified by acetone precipitation with 90% and ion exchange chromatography produced specific activity 0.25 U/mg and0.38 U/mg, and it's purity rate increased until 1.14 fold compared with former crude extract. Purifed extracelluler amilasehad an optimum activity at temperature 50°C and pH 9.0. An apparent molecular mass was between 50-75 kDa, asestimated by zimogram electrophoresis. Hydrolysis products of this enzyme on starch were maltose, maltotriose andmaltoheptaose.




Indonesian Journal of Biology

Jurnal Biologi Indonesia diterbitkan oleh Perhimpunan Biologi Indonesia. Jurnal ini memuat hasil ... see more