Deteksi Penyebaran Geografis Penyakit CVPD di Bali Utara dengan Metode Polymerase Chain Reaction

Mutia Erti Dwiastuti • Ana Triwiratno • M. Garnier • J. M. Bove
Journal article Jurnal Hortikultura • June 2003 Indonesia

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(Bahasa Indonesia, 8 pages)

Abstract

. The objective of this research was to evaluate the present status of CVPD disease on citrus trees after the citrus rehabilitation. This research was conducted along North Bali on 10-1,000 m above sea level. The technique of CVPD detection used was polymer- ase chain reaction with three specific primers (oligonucleotide) of CVPD O11, O12, and OA1 that are sensitive for both Liberobacter asiaticum and Liberobacter africanum. Identity of samples DNA amplified that were appeared on 0.7% agarose with horizontal electrophoresis. The result of observation showed of all samples from 11 symptomless samples on infected trees gave negative PCR reaction. The samples with specific symptom of CVPD gave positive PCR reaction. Based on the observation, the positive result was always obtained from orchards with altitude 10-700 m above sea level, but no reaction sign of PCR on samples from orchards above 700 m above sea level. This research re- sult can be used to arrange the control strategy of CVPD in infected areas.The detection technique can be used to deter- mine a strategy for controlling CVPD in that areas.

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Jurnal Hortikultura

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