Business combination is one of the ways used by the company in order to be more developed. Forms of business combination that often do is acquisition. The impact of acquisition will be based on the amount of stock returns and trading volume received by the acquirer. The purpose of this research is to analyze the impact of the acquisition by multinational company in Indonesia which is listed on Indonesia Stock Exchange. This type of research is hypothesis testing using non-parametric test with quantitative approach secondary data. Sample selection is done by purposive sampling method. Samples are five multinational companies in Indonesia and listed on Indonesia Stock Exchange period 2010-2012. Data analysis technique used is based on the distribution data using the Kolmogorov-Smirnov Test. This research use Wilcoxon-Signed Ranks Test. The final results showed that there is no significant differences of stock returns and trading volume of the acquirers,both before and after the acquisition.