Introduction.Fracture treatment and bone healing is a complicated process and interesting topic. There are a lot of researches in this matter until now. Fracture fixation with additional autogenous bone marrow is well known to have good fracture healing. Bone marrow contains progenitor cells that can be used to facilitate bone healing. Fracture treatment with intramedullary nailing combine with bone marrow from iliac crest or femoral head has shown to have better healing. The purpose of this study was to evaluate bone healing on the fracture site by giving the bone marrow from medulla. Materials and methods. In the present study, we explored bone healing in femoral fracture of Sprague dawley rat with intramedullary wire fixation and the application of medullary bone marrow as much as 0.5 to 1 cc from femur. The effect of given bone marrow in fracture healing was evaluated from callus formation (callus diameter, callus volume) and alkaline phosphatase level. Subjects were divided into 2 group, namely study group and control group. Each group consists of 20 white rats. The control group was treated with intramedullary wire while the study group was treated with medullary bone marrow in addition to intramedullary wire. Observation was followed until 30 days. During that time, both groups were given same environment including cage, wound treatment, food intake, water intake and temperature). Callus diameter and volume was evaluated with radiological and alkaline phosphatase level was measured from blood serum.Results.Callus diameter in study group was 34.1% larger than control group (p < 0.01). Callus volume in study group was 2.02 times larger than control group (123.77 mm3 compared to 41.23 mm3; p < 0.01). Alkaline phosphatase in study group was 23.63% larger than control group (30.56 IU/L compared to 24.72 IU/L; p < 0.01).Conclusions.We conclude that bone marrow derived from femoral reaming could increase callus formation and alkaline phosphatase level significantly. It has positive effect in femoral fracture healing to increase osteoblast activity.