. This article aims to understand the approach used by Faith Based Organization (FBO) in the tackling and handling program of HIV and AIDS, in relation to the prevention, infection, as well as the treatment in social-cultural perspective. The data collecting is done through profound interview and observation in two research areas: North-Beach Areas (Pantai Utara), Central Java and Denpasar, Bali. Both locus were chosen and compared based on the differences in socio-cultural formation, particularly in the religious background majority which in control of the social life. It is found that the involvement of both the institution and religious leaders in the countermeasures of HIV and AIDS has a strategical value either directly or indirectly. The involvement of religious organizations in these two areas, even though differ in effectiveness, scale of implementation, problems encountered, value approach, intervention and organizational attachment, and the social-cultural construction of the society has adduced changes in discourse and practices related to the HIV and AIDS issues, either in the circle of the religious leaders and the community in general. The North-Beach area in Central Java which only for the past five years go through with the countermeasures program of HIV and AIDS formally, structurally, direct, and organizational by FBO in this case Nahdlatul Ulama (NU), has the effectivity and acceptance for different people and slower that what has happened in Bali, where the FBO tends to be involved through non-organizational network.