When a woman is imprisoned, she will experience a lot of pressure. Prisons create feelings of fear and unpleasant feelings due to bad assumptions and pressures experienced such as beatings, torture, sexual harassment, poor health and very minimal facilities, besides that there is a stigma that will remain attached to someone when they are out of prison. The length of their sentence and isolation from the outside environment has a considerable psychological impact on the mental health of prisoners. The purpose of this study was to determine the anxiety level of female prisoners in prison. The sample in this study were all 30 female prisoners in prison. Using the descriptive approach method carried out in May-June 2016. The results of the study, the characteristics of respondents with the highest age were: 26-35 years totaling 15 people, highest education: high school, 16 people, the highest marital status was 26 people, the cause of entry into prison caused by drug cases totaling 21 people, and those serving the longest sentence in 1-4 years in prison amounted to 18 people. While the results of descriptive tests with SPSS on the level of anxiety of female prisoners in prison are moderate anxiety; 15 people with an average of ± std (22.93 ± 3.58) then severe anxiety consisted of 10 people, with a mean ± std (29.20 ± 5.52) and average mild anxiety ± std (14 , 60 ± 1.14). Conclusion; the importance of nurses and prison staff to pay attention to the anxiety level of women in prison because of the many problems that arise from female prisoners both physically and psychologically.