The growth pattern and condition factors of three spots gourami Trichopodus trichopterus (Pallas, 1770) from Lempake Dam, East Kalimantan, are not yet informed. These studies aimed to investigate fishes' length and body weight, sex ratio, length-bodyweight relationships, growth patterns, and condition factors. The collection of fish sampling was carried out monthly from February to May 2020. A total of 912 individual samples of sepat rawa fish were caught by 15 fish traps using a purposive sampling method at three stations. This research showed that the total length of the fishes analyzed ranged from 31.68 to 103.53 mm, while body weight ranged from 1.12 to 7.22 g. The sex ratio in males and females was 1:1.19. The regression model of the length-body weight relationships calculated was W=3x10-5L2.861 for total samples, W=5x10-5L2.710 for males, and W=2x10-5L2.977 for females. The length-body weight was obtained strong relationships in all samples and sexes, with the regression coefficient (r) ranging from 0.965 to 0.977. An isometric growth pattern and the growth coefficient “b”=2.977 was observed in females, while a negative allometric growth pattern in males and both sexes, with the growth coefficient “b”= 2.710 and “b”=2.861. The values of relative condition factor (Kn) observed for females were ranged from 1.472 to 1.555 and for males ranged from 1.024 to 1.082. The growth pattern and relative condition factor of three spot gourami in the Lempake Dam were affected by the number of fishes in gonad mature condition and body shape.