Pipe fish is a type of freshwater fish that has potential as ornamental fish. The research objective was to determine the status and habitat conditions of the Merowi River pipe fish as a member of Syngnathidae. The research was conducted from July to December 2019. The research stations were determined based on differences in existing environmental bases. Sampling had done randomly by using scoop net and umbrella fish trap. The data used include population density, age group,test Chi-Square and Principal Component Analysis (PCA). The results showed that 2 genera consisted of 3 species of pipe fish, as much 248 individu. Doryichthys deokhatoides 26 individu (0.042 Catch/Unit Efort); D. martensii 209 individu (0.34 Catch/Unit Efort); Microphis retzii 13 individu (0.02 Catch/Unit Efort). The length of the pipe fish ranged from 48.1 mm to 87.4 mm, and the ratio of male to female is 1: 1 (balanced). The presence of members of Syngnathidae in the Merowi River was effect by temperature and current velocity.