Production of Medium Chain Length Polyhydroxyalkanoates From Oleic Acid Using Pseudomonas Putida Pga1 by Fed Batch Culture

Sidik Marsudi • Irene K. P. Tan • Seng-Neon Gan • K. B. Ramachandran
Journal article Makara Journal of Technology • Nisan 2007

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Bacterial polyhydroxyalkanoates (PHAs) are a class of polymers currently receiving much attention because of theirpotential as renewable and biodegradable plastics. A wide variety of bacteria has been reported to produce PHAsincluding Pseudomonas strains. These strains are known as versatile medium chain length PHAs (PHAs-mcl) producersusing fatty acids as carbon source. Oleic acid was used to produce PHAs-mcl using Pseudomonas putida PGA 1 bycontinuous feeding of both nitrogen and carbon source, in a fed batch culture. During cell growth, PHAs alsoaccumulated, indicating that PHA production in this organism is growth associated. Residual cell increased until thenitrogen source was depleted. At the end of fermentation, final cell concentration, PHA content, and productivity were30.2 g/L, 44.8 % of cell dry weight, and 0.188 g/l/h, respectively.




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