Bandung has the highest case of dengue fever in West Java, which is 3134 cases in 2014. The spread of dengue fever can be analyzed spatially by spatial autocorrelation to find out the correlation relationship pattern between locations. The method of spatial autocorrelation was using the Moran Index method. From these methods can determine the relationship of spatial autocorrelation in the spread of dengue fever and determine spatial distribution pattern of dengue fever in Bandung. The results of spatial autocorrelation analysis shows the spatial autocorrelation in dengue hemorrhagic cases in Bandung, the one-way significance test indicates a positive autocorrelation in Dengue hemorrhagic cases in Bandung and spatial distribution pattern is the spatial distribution pattern of the cluster.