Indonesia have a large potential of geothermal resources. Geothermal resource area such as Cimanggu area, West Java Province has natural hot spring resources. These natural hot spring resource can be utilized for eco tourism and electric power plant. Natural hot spring have relatively low water temperature between 60° - 90°C and it need special technology to be utilized as electric power plant. Kalina cycle KCS34 is a cycle that utilize ammonia-water mixture to absorb energy from low temperature natural hot spring source because of the properties of ammonia-water mixture that can boiled at low temperature. The fraction of ammonia-water mixture and the condensor pressure of the Kalina cycle KCS34 can affect the turbine output power and system efficiency. Optimization simulation was held to discover the required fraction of ammonia-water mixture and condenser pressure that suitable for generating electricity on Cimanggu area, West Java Province. According to the simulation results, the optimum fraction of ammonia-water mixture is 87% and the condensor pressure at 7.6 bar which will generate 73.57 kW of turbine power with 14.85% system efficiency.