Deteksi Vibrio Harveyi dengan Metode Amplifikasi DNA pada Gen ToxR

Calvin Wijaya Johan • Dwi Putra, Sulistyo Emantoko • Ernest Suryadjaja
Journal article Keluwih: Jurnal Sains dan Teknologi • 2020

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Abstract

-Vibrio harveyi infection in aquacultures may cause death and economical loss. Rapid detection of this bacteria may prevent its dispersal in aquacultures. The goal of this research was to develop method in detection of Vibrio harveyi via amplification of toxR gene. DNA amplification was carried out with two methods, loop-mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR). Amplification with LAMP suggest optimization of either protocol or primer design was needed to prevents false positive results. Amplification with PCR yields 229 bp-length product specific to Vibrio harveyi with detection limit up to 0.526 ng.µL-1 (equals to 2.09 × 106 CFU.mL-1). Keywords: Aquaculture, LAMP, PCR, toxR, Vibrio harveyi

 

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Keluwih: Jurnal Sains dan Teknologi

Keluwih: Jurnal Sains dan Teknologi (JKST; English: Keluwih: Journal of Science and Technology) i... see more