In vivo study of ethanolic ciplukan extract (Physalis angulata) to enhance the apoptosis of tongue cancer cell. Tongue cancer is kind of oral cancer with high prevalence. Ciplukan (Physalis angulata L.) has cytotoxic activity to inhibit cancer cells proliferation and induct cell cycle arrest. This study aims to assess the potential of ethanolic ciplukan extract (ECE) as anti tongue cancer and determine the effect of ECE in increasing tongue cancer cells apoptotic injected by DMBA. Fifteen female Sprague dawley rats of approximately 80-60 gram and aged 2-3 months were divided into three groups: (1) control group; (2) DMBA+ 750 mg/kg BW ECE; (3) DMBA+1500 mg/kg BW ECE. Tongue cancer was inducted by injecting 2% DMBA 0,1ml /100 grams BW on the lateral tongue and then it was observed for 5 weeks. After that, the 750 and 1500 mg/kg BW ECE were given orally everyday for seven days. By the end of the sixth week, all the rats were sacrified and then the tongue tissue was stained using TdT-mediated X-dUTP Nick End Labeling (TUNEL) assay to see the apoptotic cells. The results showed that apoptotic index for the control group was 3,94%, treatment with 750 mg/Kg BW extract had 15,34% result meanwhile treatment with 1500 mg/Kg BW resulted in 25,5%.The result of one way ANOVA (p<0,05) test showed that ECE increased cancer cells apoptotic treated by DMBA. Thus, it is possible to conclude that ECE is effective in inhibiting the growth of tongue cancer cell by increasing apoptotic tongue cancer cells.