Extract herb pegaganconsists of flavanoid and saponin compound that can be applied as sun filter to prevent ultraviolet radiation. Herb extract pegagan and olive oil has been prepared in lotion dosage form due to its easy to use. The aims of this research was to determine varying concentrations ofphysical properties and sun protecting factor (SPF) value of sunscreen lotion. Extract herb pegagan and olive oil was obtained by maceration method using ethanol 96% as solvent. In vitro measurement of SPF used spectrophotometer Ultraviolet-visible. The SPF was calculated and plotted with concentrations using non-linear regression method. Formulation Herb.Extract pegagan and olive oilinsunscreen lotion using varying concentrations of was prepared by several ratios i.e. F1 (5%:0,1%), F2 (10%:0,1%), and F3 (20%:0,1%). Physical stability and properties of lotion were conducted i.e organoleptic, homogeneity, viscosity, spreadibility, emulsion type and pH. The results were statistically analyzed using two way analysis of variance at confidence level of 95%. The results showed that as many as extract herb pegagan and olive oil SPF values of 28,5 (ultra protection); 29,9(ultra protection); and 69 (ultra protection), respectively.