Hypercholesterolemia is a clinical condition in which total cholesterol is above the normal level (NCEP, 2001). Antihypercholesterolemic drugs such as statins and fibrates generally has side effects on myopathy (NCEP, 2001: 2491). Back to nature trends make people realize the importance of herbal therapy. The goals of the study are to determine the antihypercholesterolemic activity and the most effective dose of methanol extract of Averrhoa bilimbi Linn fruit in Rattus norvegicus.The method used is experimental with Single Blind Randomized Controlled Trial Design (RCT). The sample was randomized and divided into 5 groups consisting of positive control, negative control and three variants doses (160 mg/200 g BW, 320 mg/200 g BW and 480 mg/200 g BW). Total cholesterol levels were measured by CHOD-PAP method on day 7 (before treatment), day 17 (under hypercholesterolemia conditions) and day 31 (after treatment).Data of total cholesterol difference were analyzed using one-way ANOVA (95% confidence interval). The p-value is 0.025 (p < 0.05). Which showed that the methanol extract of Averrhoa bilimbi Linn fruit have the activity of antihypercholesterolemic. Then a post-hoc LSD test was carried out and the results showed that the most effective dose was 480 mg/200 g BW with a significance value of p= 0.006 (p < 0.05).